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Chuku, L. C.
- Analysis of Hematological and Biochemical Parameters, to Study the Ecological Relationship on Selected Species of Vertebrates
Abstract Views :515 |
PDF Views:341
Authors
L. C. Chuku
1,
A. A. Uwakwe
1
Affiliations
1 Department of Biochemistry, University of Port Harcourt, Rivers State, NG
1 Department of Biochemistry, University of Port Harcourt, Rivers State, NG
Source
Indian Journal of Drugs and Diseases, Vol 1, No 9 (2012), Pagination: 208-212Abstract
Objectives: Hematological and biochemical analysis studies of ruminant vertebrates (cow, sheep and goat) will provide us an acceptable method for understanding the ecological relationship among the vertebrates. Methods: Haematological/Biochemical studies were carried out on blood from the ruminants: Goat (Capra hircus-L), sheep (Ovis Aries dolrchra), cattle (Bos taurus) and human (Homosapiens) as control. Results: Electrophoretically, goat blood revealed three different Hb polymorphic types: HbAA (45%), HbAS (50%) and HbSS (5%). Only the 'O' Rh blood group was observed in all cases, and GST values of 4.05-6.30 I.U were obtained on the other hand. Cow Hb electrophoresis produced two patterns corresponding to the human HbAA and HbAS types. Only the 'O' Rh- blood group was obtained in all cases and GST values of 2.85-5.02 I.U were obtained, while sheep blood produced only the HbAA-type, only the 'O' blood group was identified in all case. GST activity values were in the range of 3.05-4.52 IU. Conclusion: These results could point to a definite haematological and biochemical similarity between man and ruminants and thus serves as a springboard for further genetic studies using lower vertebrate's as research models.Keywords
Haemoglobin, Glutathione-S-transferase, Genotype, ErythrocyteFull Text
References
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- Haematological and Biochemical Studies on Selected Species of Fishes
Abstract Views :339 |
PDF Views:271
Authors
L. C. Chuku
1,
A. A. Uwakwe
1
Affiliations
1 Department of Biochemistry, University of Port Harcourt, Rivers State, NG
1 Department of Biochemistry, University of Port Harcourt, Rivers State, NG
Source
Indian Journal of Drugs and Diseases, Vol 1, No 8 (2012), Pagination: 185-191Abstract
In this study the following biochemical and haematological studies on Catfish (Clarius gariepianus); Mullet (Mugi cephalus), Tilpia (Oreochromis niloticus), mudskipper (Gobi I), African sleeper (Gobi-II), were carried out; Haemmoglobin electrophorosis, ABO Blood grouping and Glutathine-S-Transferase (GST) activity. About 50% of catfish indicated the Rh.D- while thew remaining 50% indicated the Rh.D+ blood group/. The 'A' 'O' and 'AB' blood groups were detected and glutathione-S- transferase activity values of 5.64 - 7.27 I.U were obtained. Hb concentration values were of the range of 5.5 -10.7gm% blood group results gave values of 50% D+ (A,O) and 50% (AB). GST activity values of 13.50-20.10 I.U were obtained. Gobi-I and Gobi-II Hb electrophoretic pattern was similar to human HbSS blood with Hb concentration range of 1.8-5.57gm%. GST activity values of 16.97-33.08 I.U. were obtained. Haemoglobin electrophoresis of Tilapia produced multiple patterns corresponding to human HbSS, HbCC and possibly HbCD. Hb concentration values were of the range of 3.70-12.94gm% and GST opf 9.90-12.92 I.U were obtained. Only the 'O' and 'AB' blood groups were identified.Keywords
Haemoglobin, Glutathione-S-Transferase, Genotype, SerumFull Text
References
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